Silica Unbonded Silica SPE Solid Phase Extraction ColumnProvided by Shanghai Shupei Experimental Equipment Co., Ltd., Silica is an unbound silica gel filler and a highly polar positive phase adsorbent that can retain polar compounds in the sample, especially those with similar structures.

Equivalent to Agilent Bond Elut Silica and Waters Sep Pak Silica.

Silica Unbonded Silica SPE Solid Phase Extraction Columncharacteristic:

Very strong polarity retention ability

High loading capacity

Separable polar compounds with similar structures

Parameters:

Specific surface area: 480 m2/g

Particle size: 40-75 μ m

Average pore size: 70 Å

Application:

Analyze drugs, toxins, environmental pollutants, and their metabolites in biological matrices, such as pioglitazone and polychlorinated biphenyls

Separate bioactive molecules such as esters, antibiotics, bile acids, oligosaccharides, etc

Detecting fungal toxins such as fumonisin in food

Detecting preservatives in cosmetics

Silica Unbonded Silicone SPE Solid Phase Extraction Column Item Number Inquiry Table:

Related standards:

GB/T 22957-2008 Determination of nine residual glucocorticoids in pufferfish, eel and grilled eel by liquid chromatography tandem mass spectrometry

GBT5009.190-2003 Determination of Polychlorinated Biphenyls in Seafood

HJ 743-2015 Soil and sediment - Determination of polychlorinated biphenyls - Gas chromatography-mass spectrometry method

SHT 0803-2007 Determination of Polychlorinated Biphenyls in Insulating Oil by Capillary Gas Chromatography

Typical applications: Solid phase extraction and high-performance liquid chromatography for detecting impurities in milkC21H30O5
C21H30O5It is one of the residual corticosteroids in milk. The use of solid-phase extraction and high-performance liquid chromatography can quickly and reliably detect the presence of [substance] in milkC21H30O5



Material Science:
CopureTM Silica solid phase extraction column (500mg/6mL), part number COSI6500.

Steps:
1. Sample extraction
Weigh 5 g of milk sample and extract it twice with 30 mL and 25 mL of ethyl acetate. Combine the extracts, evaporate to near dryness, and dissolve in 1 mL of ethyl acetate and 5 mL of n-hexane.
2. SPE purification
Take the SPE column and activate it with 6 mL of n-hexane. Pass the test solution through the column, wash with 6 mL of n-hexane, and then wash with 6 mL of n-hexane acetone (6:4, v) to collect the eluent.
3. HPLC detection
Inject the eluent into the chromatograph, record the response values, and obtain the chromatogram of the spiked sample.

result:
For 5ppmC21H30O5The spiked milk sample had an average recovery rate of 98.6% and RSD of 0.29% (n=3).