1、 Definition of Seed Jar:

The seed tank aims to ensure the necessary amount of bacterial cells for fermentation tank cultivation. Provide the necessary nutrients for fermentation. An essential nutrient source equipment for fermentation tank production.

The main fermentation equipment includes fermentation tanks and seed tanks, each of which is equipped with raw material (culture medium) preparation, cooking, sterilization, and cooling equipment, ventilation adjustment and sterilization equipment, as well as agitators.

Tank system tank structure: All stainless steel tank body, with no dead corners inside the tank; Equipped with a dedicated sampling and discharging valve for fermentation tanks, a large angle liquid level observation mirror inside the tank, a 12V safety sight light, and temperature control PH、 DO、 Defoamer, liquid level interface, inoculation port, feeding port, exhaust port (with diaphragm valve) and multiple spare ports, circular cumulative interface, all pipeline valves are designed in accordance with GMP standards.

2、 Seed jar design structure:

2.1 Internal and external polishing, roughness Ra ≤ 0.4, tank material: 316L

2.2 Heat transfer of jacket, material SUS304;

2.3 Standard production of pressure vessels.

2.4 Tank cover structure: 1 inoculation (flame ring inoculation), 2 spare ports (for feeding, defoamer addition and acid-base addition), with foam sensor, and exhaust port with condensation device; Circular accumulation interface, tank bottom: There is one fermentation tank dedicated sampling and discharging valve (with steam sterilization), seed transfer pipeline (with steam sterilization), and tank bottom valve, designed without dead corners or liquid accumulation. The diaphragm valve controls the steam to enter the tank air system/steam ventilation:

3、 Design of Seed Jar Auxiliary System

3.1 Manual control of rotor flowmeter, adjust and control the flow rate of each gas according to process requirements.

3.2 Air system

The configuration requirements consist of a pre filter, a rotor flowmeter, an air fine filter, a diaphragm valve, and a check valve. A first stage pre filter with a filtration accuracy of 0.5um and a second stage fine filter with a precision of 0.2um are added at the main pipe. The filter element is also included

3.3 Mixing system

3.3.1 Adopting a tank top mechanical seal system, with sealing performance ** Performance mixing paddle, defoaming paddle. Variable frequency speed regulating motor (stepless speed regulation), speed regulating controller.

3.3.2 The mixing shaft is made of 316L stainless steel, with an appropriate ratio of upper and lower shafts to ensure stable and non deviating speed, and to minimize damage to the mechanical seal.

3.3.3 Fermentation specific standard mixing paddles (three levels: two levels with six flat blades, one level with diagonal blades) and defoaming paddle blades can be adjusted in degree, and different types of mixing paddles can also be replaced according to the special requirements of the fermentation process. Speed controllable range: 50-1000rpm infinite speed regulation

3.3.4 Motor: DC motor

3.4 Temperature control electric heating water cooling, circulation pump circulation temperature control, with air self escape function and water cut-off, over temperature protection function, with excellent heat exchange efficiency; Intelligent PID control ensures both the speed of temperature control and energy saving. Fermentation temperature control range: refrigeration. The water temperature is+5 ℃ -65 ℃ ± 0.2 ℃, with a resolution of 0.1 ℃.

3.5 Pressure control manual control, pressure gauge display control range: 0-0.4MPa

3.6 foam control electrode automatically detects foam or liquid level, peristaltic pump automatically adds defoamer or supplement, and measures, fully automatic PID control and alarm

3.7 Material replenishment control

Switch control, automatic flow addition and metering, fully automatic PID control, segmented control of fermentation process. The controller can achieve: analysis of replenishment curve, accumulation of added amount, display of records, and saving of batch report analysis data. Segmented control of fermentation process (automatic variable control of feeding based on fermentation time, at least 10 stages) The controller can achieve: analysis of feeding amount curve, cumulative display of feeding amount, recording of batch report, analysis of data storage

3.8 pH Control

Adopting intelligent PID control, the control accuracy is *, and it is not easy to generate fluctuations. Shielded wire detection, 2 peristaltic pump switches control the flow of acid and alkali, automatic control, and automatic metering. The electrode can be sterilized repeatedly, and the calibration function is completely calibrated by the system software.

1. Glass gel electrodes and wires resistant to temperature disinfection

2. Display range: 0.00~14.00 ± 0.01, fully automatic control range: 2.00~12.00 ± 0.02

3. Online detection, automatic alarm, equipped with peristaltic pump to automatically add acid-base solution

4. PID intelligent fuzzy control.

The controller can achieve: pH curve analysis, acid and alkali addition curve analysis, batch report analysis, acid addition cumulative display record, alkali addition cumulative display record, fermentation process sequence control (based on fermentation time, DO automatic variable control, at least 10 segments of DO control)

3.9 DO Control

Shielded wire detection can be associated and controlled with parameters such as speed, air flow rate, tank pressure, and replenishment. Wire polarographic DO electrode, measuring range of 0.1~150%, control accuracy: ± 3%, resolution: 0.1%

Fermentation process sequence control (based on fermentation time, DO automatic variable control, at least 10 stages)

The exhaust control adopts a pressure gauge to display the tank pressure, with a display range of 0-0.4MPa. Manually adjust ventilation volume with exhaust emission cooling

Condensation system, diaphragm valve controls exhaust emissions. The specially designed exhaust emission pipeline is free of liquid accumulation and bacteria contamination.

Defoaming control sensitivity 100~100000 Ω Automatic detection of foam, peristaltic pump automatically adds defoamer

Valve fittings: 1. Select high-quality stainless steel valves and fermentation specific valves, and use high-quality diaphragm valves for material valves;

2. External polished stainless steel pipes and high-quality accessories, material pipelines with sanitary grade internal and external polished pipes;

3. Including air pipelines, steam pipelines, circulating water pipelines, material pipelines, and sewage pipelines;

4. All pipe fittings and valves can be operated from the front, with a reasonable design and convenient operation;

Controller control system: BIOTECH-3000 biological process controller (see controller introduction later)

4.0 Accessories

Each tank is equipped with 4 serum replenishment bottles,

Air filter element, breathing filter, 20 meter high-quality silicone tube, SUS316L feeding needle.

Sufficient rubber stopper, O-ring, fuse, etc

Seed jar, stainless steel seed jar, bacterial culture jar
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