PlantScreenLarge scale plant phenotype imaging analysis system

PlantScreenThe large-scale plant phenotype imaging system is developed and produced by PSI company in the Czech Republic,It integrates advanced technologies such as LED plant intelligent cultivation, automation control system, chlorophyll fluorescence imaging measurement and analysis, plant thermal imaging analysis, plant near-infrared imaging analysis, plant hyperspectral analysis, automatic barcode recognition management, RGB true color 3D imaging, automatic weighing and watering system, etc. Mainly suitable for large crops such as rice, wheat, corn, cotton, etc. with a height of 40cm-120cmThe comprehensive imaging analysis of plant physiology, ecology, and morphological structure is used for high-throughput plant phenotype imaging analysis, plant stress response imaging analysis, plant growth analysis, ecological toxicology research, trait identification, and plant physiology and ecology analysis. As the world's first manufacturer to develop and produce plant chlorophyll fluorescence imaging systems, PSI company is at the forefront of technology in the field of plant phenotype imaging analysis. Its unique technology is the ability to perform large-scale chlorophyll fluorescence imaging analysis, which enablesPlantScreenBecoming the most advanced instrument and equipment for plant phenotype analysis and functional imaging analysis, measuring over 100 phenotype parameters.

System composition:

The entire system consists of an automated plant transport system, a light adaptation room, a 3D structured RGB imaging unit FluorCamThe main control big data platform consists of subsystems such as chlorophyll fluorescence imaging unit, visible near infrared hyperspectral imaging unit, short wave infrared hyperspectral imaging unit, thermal imaging unit, automatic watering and weighing unit, and a control scheduling server, application server, data server, programmable logic controller, and dedicated analysis software. All imaging analysis units and transmission systems are placed in the FS-WI large plant growth chamber with precise control of environmental conditions, ensuring the controllability and uniformity of plant sample growth conditions. The entire system can be customized according to the actual needs of users, highly meeting their actual needs.

Technical indicators:

1. Light adaptation room

· Provide uniform and stable environmental conditions, so that plants can be adjusted to the same growth state before entering the imaging measurement unit; Simultaneously perform dark adaptation or light adaptation preprocessing for chlorophyll fluorescence imaging measurement

· Light source: cool white LED (6500K)+far red LED (735nm), adjustable light intensity from 0-100%, no radiation heating effect on plants

· Size: 9120mm × 1200 × 2800mm, customizable according to user needs

· Capacity: 13 plants adapted simultaneously (height 1200mm, diameter 600mm)

2. Light curtain system

· Measurement parameters: Accurately measure the height of plants, and each imaging unit adjusts the height and position of the imaging sensor based on the measurement results

· Measurement range: 0-1250 mm

· Resolution: 2.5mm

3.3DStructural RGB imaging unit

· Maximum measured plant volume: 1010 mm x 1010 mm x 1250 mm (length x width x height)

· Height adjustment range: 0-1250 mm

· Adjustment accuracy: ± 3mm

· Resolution: 2560 × 1920, optional linear scanning sensor 4096-6144 pixels/line, 17-26000 lines/second

· Imaging time:< 10s

· Data transmission: Gigabit Ethernet

· 3DImaging specific rotating table: 0-360 ° free rotation, used for 3D model reconstruction

· Specialized imaging light source board: Cold white LED light source board, which can automatically adjust different light intensities to achieve the best imaging effect. Side light source board 1010 mm × 1250 mm, top light source board 1010 mm × 1010 mm

· Measurement parameters: leaf area, plant compactness/compactness, leaf circumference, eccentricity, leaf roundness, leaf width index, plant circle diameter, convex hull area, plant center of mass, growth height, maximum plant height and width, relative growth rate, plant fitness evaluation based on color division, leaf area difference comparison, greenness index, color contribution grading (healthy green, bright green, dark green, other colors) and other phenotypic parameters, with optional 3D reconstruction model function

4. FluorCamChlorophyll fluorescence imaging unit

· Imaging area: 35 × 35cm or 80 × 80cm

· Height adjustment range: 0-2005 mm

· Measurement light source: Orange 618nm LED

· Photochemical light source: dual color chemical light, orange 618nm LED and cool white LED, optional blue (455nm), navy blue (470nm), cyan (505nm), green (570nm), orange (605nm), etc., with a maximum light intensity of 300-2500µmol(photons)/m².s, can be upgraded to 5000µmol(photons)/m².s

· Saturated light source: cool white LED, maximum light intensity of 3000µmol(photons)/m².s, can be upgraded to 10000µmol(photons)/m².s

· Far red light source: 735nm LED

· 7Position filter wheel (optional): Install filters of different frequency bands and use corresponding light sources to measure fluorescent proteins such as GFP

· Imaging Sensor: High Resolution TOMI-2 CCD Sensor

a) Line by line scanning CCD

b) Maximum image resolution: 1360 × 1024 pixels

c) Time resolution: up to 20 frames per second at the highest image resolution

d) A/D conversion resolution: 16 bits (65536 grayscale levels)

e) Pixel size: 6.45 µ m × 6.45 µ m

f) Operation mode: 1) Dynamic video mode, used for measuring chlorophyll fluorescence parameters; 2) Snapshot mode, used for measuring fluorescent proteins such as GFP and fluorescent dyes

g) Communication mode: Gigabit Ethernet

· Measurement program and measurement parameters:

a) Fv/FmMeasurement parameters include Fo, Fm, Fv, QY, etc

b) KautskyInduction effect: Fluorescence parameters such as Fo, Fp, Fv, Ft_Lss, QY, Rfd, etc

c) Fluorescence quenching analysis: Fo, Fo’, Fs, Fm, Fm’, Fp, FtDn, FtLn, Fv, Fv'/ Fm', Fv/ Fm ,Fv',Ft,ΦPSII, NPQ_Dn, NPQ_Ln, Qp_Dn, Qp_Ln, qN, qL, QY, QY_Ln, Rfd, ETR Waiting for over 50 parameters

d) Light response curve LC: Fluorescence parameters such as Fo, Fm, QY, QYLn, ETR, etc

5. Visible Near Infrared Hyperspectral Imaging Unit

· Imaging area: top 1010mm x conveyor belt length, side 1250mm x conveyor belt length

· Height adjustment range: 0-1250 mm

· Adjustment accuracy: ± 3mm

· Imaging wavelength range: 380-1000nm

· Imaging sensor: Push scan linear scanning sensor, equipped with a dedicated scanning light source

· Pixel dispersion: 0.98nm

· Spectral band: 675

· Frame rate: 12-236

· Measurement parameters: Reflectance spectral imaging maps and full spectrum curves for each band, and automatic calculation of the following vegetation indices: Normalized Difference Vegetation Index (NDVI), Simple Ratio Index (SR), Improved Chlorophyll Absorption Reflectance Index (MCARI), Improved Chlorophyll Absorption Reflectance Index (1MCAI1), Optimized Soil Adjusted Vegetation Index (OSAVI), Greenness Index (G), Conversion Carotene Index (TCARI), Triangular Vegetation Index (TVI), ZMI Index, Simple Ratio Pigmentation Index (SRPI), Normalized Demethylation Index (NPQI), Photochemical Vegetation Reflectance Index (PRI), Normalized Chlorophyll Index (NPCI), Carter Index, Lichtenhaler Index, SIPI Index, Gitelson Merzlyak Index, etc

6. Short wave infrared hyperspectral imaging unit

· Imaging area: Top 1010mm x conveyor belt length, side 1250mm x conveyor belt length, can share one imaging room with visible near-infrared hyperspectral imaging unit

· Height adjustment range: 0-1250 mm

· Adjustment accuracy: ± 3mm

· Imaging wavelength range: 900-2500nm

· Imaging sensor: Push scan linear scanning sensor, equipped with a dedicated scanning light source

· Pixel dispersion: 0.75nm

· Spectral band: 200

· Frame rate: 60-100

· Measurement parameters: Reflection spectrum imaging and full spectrum curve of each band, non-destructive measurement of changes in overall and different parts of plant moisture content

7. HeatImaging unit

· Height adjustment range: 0-1250 mm

· Adjustment accuracy: ± 3mm

· Imaging sensor: focal plane array microcalorimeter, resolution 640 × 480 pixels, temperature range -20-120 ℃, thermal sensitivity<0.05 ℃ @ 30 ℃/50mK, focal length range 40-50 cm

· Specialized imaging light source: Cold white LED light source board, used to provide a stable thermal environment for measuring plants, with a maximum light intensity of 1000 µ mol (photons)/m ². s, adjustable from 0-100%

· Measurement parameters: actual temperature at each point of the plant, distribution map of plant surface temperature

8. Automatic watering and weighing unit

· Measurement parameters: actual weight, watering volume, final weight, relative weight of each culture pot

· Operation instruction: Pour the same amount of water (absolute grams or percentage of actual weight) into each culture pot; Maintain relative weight; Customize the irrigation amount for each cultivation pot to simulate different drought or waterlogging stresses; Optional nutrient supply system can quantitatively supply plant nutrients (such as nitrogen fertilizer, etc.) with irrigation, simulating different nutrient deficiency conditions; Automatic zero calibration before weighing, and automatic recalibration can also be performed using known weight items (such as weights)

· Maximum carrying capacity: 10000g for large plants, 500g for small plants, safe load of 200%

· Weighing accuracy: ± 2g for large plants, ± 0.2g for small plants

· Pouring unit: the flow rate is 3L/min, and the height of the pouring gate can be automatically adjusted up and down, back and forth to ensure the best pouring position

9. Automated Plant Conveyor System

· Conveying plant size: maximum height of 1500mm, maximum diameter of 1000mm

· Synchronized transmission of cultivated plant quantity: 176 large individual plants, 22 plants per column, for a total of 8 columns; 3520 small plants, 176 sample trays, with 20 plants per tray; Customizable according to user needs

· Cultivation pot: UV resistant polypropylene material, large cultivation pots with a diameter of 9 inches or 6 inches, or small plant trays with a size of 35 × 30cm

· Circular Conveyor Channel: Three phase asynchronous motor with gearbox, power of 200-1000W, maximum load of 500kg, speed of 150mm/s, conveyor belt material made of UV resistant and highly durable PVC

· Mobile control system: Central processing unit CJ2M-CPU33; Maximum digital input/output of 2560 points; The maximum input/output unit is 40; Temperature sensor Pt1000, Pt100，PTC； PLC communication 100Mbps Ethernet; OMRON MECHATROLINK-II maximum 16 axis precise positioning

· QR code reader: automatically reads the QR code on each sample tray; Identification distance 2-20cm; Communication RS485; Can read 1D, 2D, and QR codes; Equipped with LED light source for easy identification in low light conditions

· Environmental monitoring sensors: temperature and humidity sensor, PAR photosynthetically active radiation sensor, LM500 spectral sensor

· The main control system automatically regulates the measurement time, measurement sequence, measurement parameters, watering time, and watering amount of each sample tray. The entire process of sample operation from the measurement unit to the culture room can be fully controlled automatically, and all experimental measurement work can be completed according to the preset program without human supervision.

10. FS-WILarge plant growth chamber

· Size: 11m × 5.5m; Customizable according to user needs

· Cultivation area: 60.5m²Can be customized according to user needs

· Light source: Cool white LED (6500K)+Far red LED (735nm), other light sources such as RGB tri color light source board can be customized, with 0-100% control, dedicated light source cooling airflow channel,Programmable simulationChanges in natural light environment such as diurnal cycles, sunrise and sunset, and various other arbitrary changes

· Maximum homogeneous light intensity: 1000 µ mol (photons)/m ². s, customizable for higher light intensity

· Temperature control range: 10 ℃ -40 ℃ (the control effect depends on the light intensity and ambient temperature, with a maximum room temperature of 30 ℃), and a larger temperature control range can be customized,Programmable simulationDay night cycle changes, temperature fluctuations such as sunrise and sunset in nature, and various other arbitrary changes

· Moisture control range: 40-80% ± 7% (control effect depends on light intensity),Programmable simulationChanges in natural humidity such as diurnal cycles, sunrise and sunset, and various other arbitrary changes

· Ventilation flow rate: 1200L/hour, no wind effect, laminar gas exchange, dedicated ventilation airflow channel

· Internal materials: specialized biocompatible materials

Daegu Gyeongbuk Institute of Science and Technology in South KoreaPlantScreenFS-WI Large Plant Growth Chamber Equipped with Phenotypic Imaging System

11. Main control big data platform

· Composition: Control and scheduling server, application server, data server, programmable logic controller, and dedicated analysis software, etc

· Automatic control and analysis function: It has user-defined and editable automatic measurement programs (protocols), which automatically complete all experiments according to the user's set program. The data results are automatically stored and analyzed, and the analyzed data results can be automatically displayed in the form of dynamic curves.

· MySQLA database management system that can handle large databases with millions of records, supports multiple storage engines, and automatically stores relevant data in different tables in the database

· Plant code registration function: including storing plant identification codes, tray identification codes, etc. in the database, automatically extracting and reading barcodes or RFID tags during measurement

· Touch screen operation interface, online display of plant tray quantity, light intensity, analysis and measurement status and results, etc., easily and completely control all mechanical components and imaging workstations through software

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