PlantScreen High throughput Plant Phenotype Imaging Analysis Platform (Conveyor Version) (II)

10.Root imaging analysis

·RhizoTron root window technology, fully automatic imaging analysis, standard root window 44x29.5x5.8cm (height x width x thickness)

·Not only can it perform imaging analysis on root systems, but it can also perform imaging analysis on aboveground seedlings (shoots) with a maximum height of 50cm

·New generation CMOS sensor with a resolution of 12.3MP

·Uniform LED light source

·3-layer positioning (top, middle, bottom) root irrigation system (optional), with 3 water tanks operating independently

·Measurement parameters include: root depth (or height), root cap width, height to width ratio, root cap area, root cap compactness, total root length, axial symmetry, root tip number, root node number, etc

11.Automatic watering and weighing unit

·Measurement parameters: actual weight, watering volume, final weight, relative weight of each culture pot

·Operation instruction: Pour the same amount of water (absolute grams or percentage of actual weight) into each culture pot; Maintain relative weight; Customize the irrigation amount for each cultivation pot to simulate different drought or waterlogging stresses; Automatic zero calibration before weighing, and automatic recalibration can also be performed using known weight items (such as weights)

·The watering amount, date, and time of each cultivation pot can be programmed and recorded separately to create different drought stress gradients, and seamlessly integrated with the phenotype big data of the entire system for analysis

·Weighing accuracy: ± 2g for large plants, ± 0.2g for small plants

·Pouring unit: the flow rate is 3L/min, and the height of the pouring gate can be automatically adjusted up and down, back and forth to ensure the best pouring position

12.Automated Plant Conveyor System

·Transfer plant size: up to 200cm according to customer requirements

·Conveyor capacity: 50 pots of plants (1000 small plants), expandable to larger capacities such as 100 pots, 200 pots, 400 pots, etc; The phenotype analysis flux depends on different protocols, and it takes 100 minutes to complete the phenotype analysis of the entire system load plant samples. It can be randomly transferred to the imaging room for imaging analysis and random watering

·Cultivation Basin: Made of UV resistant polypropylene material, standard 5L (24cm diameter) cultivation basin can be used with an adapter. 3L cultivation basin can be rotated 360 degrees

·Equipped with a manual loading loop for manual sample analysis experiments, group experiments, etc. in system standby mode

·Equipped with laser plant height measurement and monitoring system and laser positioning system

·Circular Conveyor Channel: Three phase asynchronous motor with gearbox, power of 200-1000W, maximum load of 500kg, speed of 150mm/s, conveyor belt material made of UV resistant and highly durable PVC

·Mobile control system: Central processing unit CJ2M-CPU33; Maximum digital input/output of 2560 points; The maximum input/output unit is 40; Temperature sensor Pt1000, Pt100，PTC； PLC communication 100Mbps Ethernet; OMRON MECHATROLINK-II maximum 16 axis precise positioning

·RFID tags and QR plant identification system automatically read the two-dimensional code on each sample tray; Identification distance 2-20cm; Communication RS485; Can read 1D, 2D, and QR codes; Equipped with LED light source for easy identification in low light conditions

·Environmental monitoring sensors: temperature and humidity sensors, PAR photosynthetically active radiation sensors

·The main control system automatically regulates the measurement time, measurement sequence, measurement parameters, watering time, and watering amount of each sample tray. The entire process of sample operation from the measurement unit to the culture room can be fully controlled automatically, and all experimental measurement work can be completed according to the preset program without human supervision.

13.Master control phenotype big data platform

·Composition: Control and scheduling server, client application server, data server, programmable logic controller, and professional analysis software, with a data capacity of 12TB

·Automatic control and analysis function: It has user-defined and editable automatic measurement programs (protocols), which automatically complete all experiments according to the user's set program. The data results are automatically stored and analyzed, and the analyzed data results can be automatically displayed in the form of dynamic curves.

·MySQL database management system, capable of handling large databases with millions of records, supports multiple storage engines, and automatically stores relevant data in different tables in the database

·Plant code registration function: including storing plant identification codes, tray identification codes, etc. in the database, automatically extracting and reading barcodes or RFID tags during measurement

·Touch screen operation interface, online display of plant tray quantity, light intensity, analysis and measurement status and results, etc., easily and completely control all mechanical components and imaging workstations through software

·All measurements can be performed using the default program, or custom workflows can be created through development tools, or manually operated to turn on or off LED light sources, RGB imaging, chlorophyll fluorescence imaging, hyperspectral imaging, infrared thermal imaging, 3D laser scanning, weighing and watering, etc

·Leaf tracking module, which can continuously track and monitor the growth, changes, and other aspects of leaves

·3D projection technology can be used to construct 3D models through high-resolution RGB lenses or laser scanning. Through projection technology, data obtained from other sensors such as chlorophyll fluorescence, infrared thermal imaging temperature data, near-infrared data, hyperspectral data, etc. can be projected onto the 3D model for comparative analysis

·Allow users to remotely access data processing, download and change experimental design through the Internet

·All measured data is transparent and traceable

·Equipped with user permission grading function to prevent other personnel from accidentally affecting the experiment

·Manufacturer remote fault diagnosis, software lifetime free upgrade

Execution standards:

·CE certification standard

·CSN EN 60529 Protection Level Standard

·CSN 33 01 65 Conductor Side Identification Standard

·CSN 33 2000-3 Basic Characteristics Standard

·CSN 33 2000-4-41ed. 2 Electric shock protection standard

·CSN 33 2000-4-43 Power Overload Protection Standard

·CSN 33 2000-5-51ed. 2 General Rules and Standards

·CSN 33 2000-5-523 allowable current standard

·CSN 33 2000-5-54ed. 2 Grounding and Protective Conductor Standard

·CSN EN 55011 Scope and methods for measuring electromagnetic interference in industrial, scientific and medical equipment

·2006/42/EG Machinery Directive Standard

·73/23/EEG Low Voltage Command Standard

·2004/108/EG Electromagnetic Compatibility Directive Standard

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Attachment: Other phenotype analysis platforms:

1. FKM multispectral fluorescence dynamic microscopy imaging system

The image on the right is quoted from《Nature Plants》2016, Photonic multilayer structure of Begonia chloroplasts enhances photosynthetic efficiency by Heather M. Whitneyetc.

2. PlantScreen-R mobile phenotype analysis platform (bottom left image): used for field plant chlorophyll fluorescence imaging analysis, RGB imaging analysis, infrared thermal imaging analysis, 3D laser scanning measurement analysis, etc

3. PlantScreen desktop and mobile plant phenotype analysis platform (see the upper right figure)

1) 3D RGB color imaging analysis

2) FluorCam Chlorophyll Fluorescence Imaging Analysis

3) FluorCam multispectral fluorescence imaging analysis

4) Hyperspectral imaging analysis

5) Infrared thermal imaging analysis

6) PAR absorption/NDVI imaging analysis

7) Near infrared 3D imaging analysis

4. PlantScreen strip phenotype analysis platform

5. PlantScreen Plant Phenotype 3D Automatic Scanning Imaging Analysis Platform