Femtosecond Fluorescence Upconversion&TCSPC Extended HARPIA-TF-Fornitore globale

2nd Floor, Building 5, Dongsheng International Entrepreneurship Park, No.1 Yongtaizhuang North Road, Haidian District, Beijing

※Combining femtosecond upconversion with TCSPC measurement takes up less space
※Simple operation and easy daily maintenance
※ serve asHARPIA-TAThe attachment is used as an independent unit
※Easy switching between fluorescence upconversion and TCSPC mode
※Operating at a frequency of 50-1000 kHzPHAROSandCARBIDESeries laser compatible
※Simulated PMT detector option for fluorescence upconversion
※Automatic spectral scanning and calibration of upconversion crystals and prisms
※Measure fluorescence dynamics in the femtosecond to microsecond range
※Fully control the following parameters of the pump beam:
Polarization (using Berek polarization compensator)
- Intensity (using manual or automatic continuously variable neutral density filters)
- Gate delay (using optical delay lines)
※Spectral resolution fluorescence detection using a monochromator
※WithHARPIA-TAWhen used in conjunction with the host, a single monochromator can be used for time-resolved absorption and fluorescence measurements without the need to replace the detector. Other monochromator options are also available, such as the dual subtraction monochromator for higher TCSPC time resolution

HARPIA-TF is a time-resolved fluorescence measurement module that combines fluorescence upconversion and TCSPC technology. In fluorescence upconversion, the signal from the sample is mixed with femtosecond gate pulses in a nonlinear crystal to achieve high temporal resolution, which is limited by the duration of the gate pulses and ranges from 250 fs. For fluorescence decay times exceeding 150 ps, the instrument can be used in time-dependent single photon counting (TCSPC) mode to measure dynamic trajectories in the range of 200 ps-2 μ s. The HARPIA-TF module supports Becker&Hickle TCSPC devices and detectors.

The combination of these two time-resolved fluorescence techniques can measure spectral resolved fluorescence attenuation in the femtosecond to microsecond range.

By using high repetition rate PHAROS or CARBIDE lasers, fluorescence kinetics can be measured while exciting the sample with low pulse energy up to several nanojoules.

Product Core Words】

TA transient absorption spectroscopy system, femtosecond/nanosecond/picosecond pump detection, fluorescence upconversion spectroscopy, ultrafast time-resolvedPump detection system

Related products

Click here to view ☞:Femtosecond ultrafast pump detection spectroscopy system HARPIA

Click here to view ☞:Ultra fast transient absorption spectrometer HARPIA-TA

Click here to view ☞:Third beam transmission HARPIA-TB

Click here to view ☞:Microscopic module

[Specification Parameters]

Upconversion mode

wavelength range	300 – 1600nm
Wavelength Resolution	Due to the bandwidth limitation of the gating pulse, it is generally around 100 wavenumbers
Delay range	4ns, 6ns, 8ns
Delay order	4.17fs, 6.25fs, 8.33fs
Temporal resolution	<1.4 x pump or probe pulse width (depending on longer pulse width), standard PHAROS laser pulse width is 420fs
Signal to Noise Ratio	100: 1.5, assuming a cumulative time of 0.5 seconds per point

TCSPC mode

parameter	numerical value
TCSPC module	Becker&Hickl SPC 130, Fully integrated into software
Detector control	Becker&Hickl DCC 100
Photomultiplier tube	Becker&Hickle PMC 100 1 Standard
wavelength range	300 – 820nm
Inherent time resolution	<200ps
Time resolution with monochromator	<1.2ns
Signal to Noise Ratio	<100:1, assuming each trace has a 5-second accumulation time

Spectrometer size:275 (width) × 571 (length) × 183 (height) mm

Left: HARPIA optical layout for fluorescence upconversion measurement

Right:Fluorescence kinetics of DCM laser dye in solution obtained using HARPIA-TF in fluorescence upconversion mode

Outline drawing of HARPIA system with HARPIA-TB and HARPIA-TF modules

Left:The principle of Time Dependent Single Photon Counting (TCSPC), right:Principle of time-resolved fluorescence upconversion