Can detect total coliforms, Escherichia coli, and fecal (heat-resistant) coliforms and provide quality control. Let you have no worries when developing new methods. At the same time, the test results can also serve as a reference for method expansion and laboratory certification.

Enzyme substrate based coliform detection system

• Provide positive and negative bacterial strains for testing the accuracy and reliability of the entire experiment

• Enzyme substrate method reagents can simultaneously detect total coliforms and Escherichia coli or fecal (heat-resistant) coliforms within 24 hours

• No need to operate in a sterile room

  The quality control kit includes:

• 200 enzyme substrate method reagents

• JC-51 type 51 hole quantitative disk, 100 pieces

• JC-97 type 97 hole quantitative disk, 100 pieces

• 200 JC-100 sterile sampling bottles

• 1 set of quality control samples

• One 97 well positive colorimetric disc

  Quantitative testing:Used in conjunction with 9900Z intelligent programmable quantitative sealing machine

  Positive result:The yellow color of the sample indicates a positive total coliform or fecal (heat-resistant) coliform, while the yellow color and fluorescence indicate a positive Escherichia coli. No change to negative result

  Testing steps:

• Qualitative testing

• Step 1: Add reagents to the sample and incubate for 24 hours.

• Step 2: Read the result:
  Colorless=Negative
  Yellow=Total coliform bacteria
  Yellow/Fluorescent=Escherichia coli

• quantitative detection

• Step 1: Add the reagent to the sample.

• Step 2: Pour into a 51 well quantitative plate®(Counting range from 1 to 200) or 97 well quantitative disc®(Counting range from 1 to 2419)

• Step 3: In the programmable quantitative controlSeal on the sealing machine and incubate in a 35 ± 0.5 ℃ incubator for 24 hours (temperature requirements may vary depending on regulations in other countries).
  If fecal (heat-resistant) Escherichia coli is detected, it should be cultured in a 44.5 ± 0.2 ℃ incubator for 24 hours.

• Step 4:51 hole quantitative disk - reading result:
  Yellow hole=total coliforms/fecal (heat-resistant) Escherichia coli
  Yellow/fluorescent hole=Escherichia coli
  Count the number of positive wells and refer to the MPN table of the 51 well quantitative disk

• 97 hole quantitative disk - reading result:
  Yellow hole=total coliforms/fecal (heat-resistant) Escherichia coli
  Yellow/fluorescent hole=Escherichia coli
  Count the number of positive wells and refer to the MPN table of the 97 well quantitative disk