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In the analysis process of GPC, the retention time is proportional to the logarithm of the molecular weight. Therefore, even a small difference in retention time (1%) can cause a huge difference in molecular weight calculation results (10%).
Many foreign manufacturers use circular gear transmission to transport solvents, because the circular motion of the circular gear becomes a linear motion of the plunger rod, which still has a slight discontinuity, resulting in pulsation of the infusion. They can only use buffers and variable stroke to compensate for the pulsation of their pumps. However, the design defects of the pump cannot be completely solved by remedial measures, and system noise will still be generated; The use of buffers also leads to a large lag volume in the system. In the early stages of instrument use, it may not have a significant impact on analysis accuracy, but the longer it is used, the more pronounced the inaccuracy of the analysis results becomes; These remedial measures will greatly reduce the service life of the plunger rod and sealing ring, so it is necessary to replace the sealing ring frequently, which is also a considerable expense in the future, and the key is to affect work efficiency. The special heart-shaped gear transmission driven infusion pump designed by Waters Corporation can have almost negligible pulsation without the need for an external buffer. The advantage of this is that the system noise is very low, the signal-to-noise ratio is good, and it can maintain good precision for a long time, ensuring excellent reproducibility; In addition, there will be no wear on the plunger rod and sealing ring, with a long service life, greatly saving customers' subsequent maintenance and usage costs.
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